The present study endeavors to investigate and compare the glycemic indices of date sugar and commercial sugar in healthy adult participants, with a particular focus on assessing their respective effects on blood glucose levels over a two-hour postprandial period. The objective of the research was to compare the glycemic index between date sugar and commercial sugar in students of PAMO University of Medical Sciences. The experiment was conducted involving a sample size of n=30. Each participant underwent two separate trials, where they consumed a controlled quantity of date sugar and commercial sugar dissolved in a standardized solution at different times. Capillary blood samples were collected at fasting state and at 30 minutes intervals after sugar consumption for 2 hours. The samples were measured for glucose level using glucometer (a strip method). The results showed that the mean glycemic index (fasting = 5.0±0.4; 30 minutes = 6.7±0.6; 60 minutes = 5.9±0.6; 90 minutes = 5.4±0.6 and 120 minutes = 4.9±0.5) of date sugar in the 2 hours period was significantly different (p-value<0.05). The mean glycemic index (fasting = 4.7±0.5; 30 minutes = 6.6±0.9; 60 minutes = 6.0±0.8; 90 minutes = 5.4±0.6 and 120 minutes = 5.2±0.5) of commercial sugar in the 2 hours period was significantly different (p-value<0.05). Comparing the glycemic index between both sugar revealed no significant difference (p-value>0.05) until at 2hours where there was a significant decrease (Date sugar = 4.9±0.5; commercial sugar = 5.2±0.5; p-value<0.05) in glycemic index in date sugar. This study has thus shown that although both sugars similar impact on glycemic index but at 2 hours, date sugar had lower glycemic index than the commercial sugar.